عنوان مقاله [English]
In order to evaluate drought tolerance and genetic variation in barley genotypes based on morpho-physiological traits and molecular markers, an experiment carried out in split plot based on randomized complete block design (RCBD) in three replications. Main factor was the different levels of irrigation included continuous irrigation as control, 2/3 and 1/3 continuous irrigation and secondary factor included 18 barley genotypes. Analysis of variance showed considerable variation among genotypes for the measured traits. Also, the Genotype×Stress interaction had significant effect on grain number per spike, protein content, proline content, carotenoid content, chlorophyll a, b and yield. In ISSR markers analysis 9 primers used that were totally produced 61 bands, where 50 bands were polymorphic. The mean PIC and MI indices were obtained for all primers were 0.46 and 2.6, respectively. High value of these indices showed high performance of certain primers for synchronized analysis of more bands. At current study, primer 2 with PIC index of 0.57 was the best primer for evaluation of genetic variation amon the studied barley genotypes. At well watered level (control), the highest significant correlations found between markers and morphological traits related to P5L5 and the highest correlation among markers and physiological traits were related to P16L2 markers. At 2/3 well watered condition the highest significant correlations between markers and morphological were related to P5L5 and P9L5, and also among marker and physiological traits belonged to P13L4 and P5L5 markers. According to cluster analysis, the genotypes were classified into four clusters. The first clusters included three genotypes Licivi, Sahand and FA1-2. Also, the genotypes F-GRB-84-11,F-GRB-85-5,PALLIDUM48//NORDIC…،.GLORIA-BAR/COPAL//.., Dasht and Sahra were located in second cluster and third cluster containing genotypes CHAMICO/TOCTE// CONGONA (a), CHAMICO/TOCTE//CONGONA(d) CONGONA/BORR and the other genotypes were located in the fourth cluster. Overall of indices and studied traits, it seems that among the studied genotypes the genotypes STIPA/PETUNIA//KOLLA/BBSC (a) and CHAMICO/TOCETE//CONGONA (a) for irrigated areas and the Sahand genotype for rainfed areas are suitable genotypes.